Acinetobacter baumannii(Acinetobacter baumannii) is an opportunistic pathogen that can cause a wide range of infections, including meningitis, bloodstream infections, ventilator-associated pneumonia, and urinary tract infections. In 1970, whenAcinetobacter baumanniiWhen the infection is first detected, most isolates are susceptible to most antibiotics. Currently, the emergence of multidrug-resistant bacteria (MDR)Acinetobacterstrains have become a serious threat to global health (Aliakbarzade et al., 2014; Azimi et al., 2015). Typically, MDR strains are resistant to 3 or more classes of antibiotics, including fluoroquinolones, third-generation cephalosporins, aminoglycosides, and carbapenems (Azizi et al., 2016; Kooti et al. , 2015). Reports over the past two years show a 74% increase in the prevalence of MDRAcinetobacter baumanniiInfection in Iran (Pourhajibagher et al., 2016). Recently, a new group of antibiotic resistanceAcinetobacterThere have been infections that are not treatable with carbapenems. Carbapenemase production is the most common mechanism of carbapenem resistanceAcinetobacter baumannii(Kuan et al., 2015). These enzymes are divided into nonmetal carbapenemases (classes A and D) and metallocarbapenems (class B). Of these, the carbapenem-hydrolyzing class D β-lactamases known as oxacillinases are by far the most prevalent type of carbapenem resistance.Acinetobacter baumannii(Evans and Amis, 2014). So far, six OXA carbapenemase families have been identified, including OXA-51-like, OXA-40/24-like, OXA-23-like, OXA-143-like, OXA-58-like and OXA-48-like (Nowak and Paluchowska, 2016; Van Bressem et al., 2001). Nevertheless, metallo-β-lactamases (MBLs) areAcinetobacter baumanniiIn contrast to OXA-type carbapenemases, these enzymes are able to hydrolyze almost all available β-lactam antibiotics. Four MBL types including SIM, VIM, IMP and most recently NDM are known so farAcinetobacter baumannii(Abbott et al., 2013). thisbraNDM-1The gene was first reported from India and then rapidly spread to other countries (Bedeni-ç et al., 2014; Poirel et al., 2011). According to research,AcinetobacterStrain carrybraNDM-1Genes confer resistance to almost all antibiotics (Muir and Weinbren, 2010). thisbraNDM-1The gene encodes the carbapenemase NDM-1, which hydrolyzes and inactivates all β-lactam antibiotics, including carbapenems except aztreonam (Yong et al., 2009).
It has been shown that the production of the NDM-1 enzyme depends on the presence of a strong promoter such as the ISAba125 gene (Poirel et al., 2011; Toleman et al., 2012). most,branoDM-1The gene is located between the two copies of ISAba125 as a Tn125 transposon (Poirel et al., 2012). Tn125 can be inserted at many gene locations as direct repeats on chromosomes or plasmids. According to previous research, inAcinetobacterisolated from Europe and North Africa,braNDM-1The gene is chromosomal, whereas in isolates from China and India it was mainly detected on plasmids (Hu et al., 2012; Jones et al., 2014; Wang et al., 2012; Zhou et al., 2011).
This study aimed to assess the frequency of carbapenem resistanceAcinetobacter baumanniiBy detecting the strainbraOXA-23andbraNDM-1drug resistance geneAcinetobacter baumanniiClinical isolates obtained from the Imam Reza Hospital in Tabriz, Northwest Iran.
Sampling and bacterial identification
In this cross-sectional study (from 2014 to 2015), clinical samples (blood, tracheal aspirates, bronchial washings, sputum, abscesses, wounds, catheters, ascites, and urine) were collected from referral to Iraqi patients. Tabriz Hospital Tabriz Hospital collected individuals from several wards in Mam Reza including Intensive Care Unit (ICU), Infectious Diseases, Traumatology, Neurology, Internal Medicine, Chest, General Surgery and Orthopedic wards. The patient's clinical data were recorded prior to sample collection.
The obtained samples were cultured in
Patient and Bacteria Isolation
A total of 100 clinical isolatesAcinetobacter baumanniiObtained from patients aged 10 to 80 years. Table 1 summarizes the demographics of the inpatients, including sex, age, sampled wards, and clinical specimen types.
thisAcinetobacter baumanniiClinical isolates were tested by standard microbiological and biochemical assays. Molecular detectionAcinetobacter baumanniiIsolates are made by amplifyingbraOXA-51like gene, shown as a 640bp band
Rising prevalence of nosocomial infections due to multidrug resistanceAcinetobacter baumanniiStrains have become a serious problem in clinical settings (Liu et al., 2014; Oh et al., 2013). According to recent studies, the rate of carbapenem resistance in Iran has increased from 51.1% in 2007 to 98% in 2017 (Bagheri Josheghani et al., 2015; Moradi et al., 2015). Therefore, continuous monitoring of antibiotic resistance patterns is critical in order to provide effective treatment policies.
Based on our
Our results show a high incidence ofbraOXA-23like genes and emergencebraNDM-1Gene as marker of resistance to nearly all antibioticsAcinetobacter baumanniistrains in the study area.
at the rate ofbraNDM1Resistance is recorded at 1%Acinetobacter baumanniiIsolated in the study area. Studies in larger populations may be necessary to determine the role of this important resistance mechanism inAcinetobacter baumanniiQuarantined in Iran.
New Delhi - Metallo-beta-lactamases
- Acinetobacter baumannii
Ethics approval and consent to participate
The study was performed after obtaining ethical approval from the Ethics Committee of Tabriz Medical University, Tabriz, Iran (reference number: IR.TBZMED.REC.1396.811). Data and swab samples were collected after written consent and a brief explanation of the importance of the study to the participant.
Agree to publish
This study received no specific funding from a funding agency.
HKh designed the study and collected the samples. LR and SF drafted the work and wrote the manuscript. HF interprets the data. BN, AT participated in the research analysis. Final manuscript read and approved by all authors.
Availability of data and materials
All data generated or analyzed in this study are included in this published article.
Statement of Competing Interests
The authors have no conflicts of interest.
This study was supported by a grant from the Biotechnology Research Center (No. 64354),tabriz medical university, Tabriz, Iran.
Produced bla isolated from clinical samples around the world
NDMPrevalence of Acinetobacter baumannii strains; systematic review
2023, Gene Reports
A discussion was held to resolve disagreements among the reviewers. Initial searches identified 3583 articles in electronic databases, of which, based on the screening process shown in Figures 1 and 2. 1, 67 articles (Table 1) were eligible for final analysis (Dally et al., 2013; Hasan et al., 2014; Islam et al., 2012; Chen et al., 2011; Tran et al., 2017; Saranathan et al. Solanki et al., 2013; Bernabeu et al., 2017; Mathlouthi et al., 2016; Shamsuzzaman, 2017; Leungtongkam et al., 2018; Bakour et al., 2014; Rafei et al., 2014; Rocha et al., 2019a; Pritsch et al., 2017; Kim et al., 2016; Yagoubat et al., 2017; Sjölander et al., 2014; Mishra et al., 2013; Salloum et al., 2018 ; Banerjee et al., 2018; Gomaa et al., 2017; Chatterjee et al., 2016; Khorsi et al., 2015; Ghebremedhin et al., 2016; Kleinkauf et al., 2014; Memish et al., 2015; Wassef et al., 2016; Jose et al., 2017; Ramoul et al., 2016; Hadjadj et al., 2018; Kumar and VinodKumar, 2017; Srirattakarn et al., 2017; Uwingabiye et al., 2017; et al., 2017; Solanki et al., 2014; Shenoy et al., 2014; Rahman et al., 2018; Jaidane et al., 2018; Patil et al., 2019; Tada et al., 2019; Ogbolu et al., 2020; Rao et al., 2020; Rahbarnia et al., 2020; Sharma et al., 2020; Fernandez-Cuenca et al., 2020; Kongthai et al., 2021; Vijayakumar et al. , 2020; Gomez-Gamboa et al., 2019; Al-Hassan and Al-Madboly, 2020; Li et al., 2019; Callad et al., 2020; Kalal et al., 2020; Al-Hamad et al., 2020; Anane et al., 2020; Kumar et al., 2019; Tada et al., 2020; Wang et al., 2019; Eslami et al., 2019; Al-Hassan et al., 2019; Villacís et al., 2019; Rossi et al., 2021; Abouelfetouh et al., 2019; Benmahmod et al., 2019; Lukovic et al., 2020; López-Leal et al., 2019; Jain et al., 2019). The reasons for excluding studies based on title, abstract and full text are shown in Figure 1.
An increasing number of nosocomial infections are associated withAcinetobacter baumannii(Acinetobacter baumannii) lead to this bacterium becoming a more clinically significant organism. Emergence of NDM-type carbapenemases has aroused great concern in the medical communityAcinetobacter baumanniiStrains that hinder the effectiveness of nearly all antimicrobials. This systematic review aimed to assess the global prevalence of carbapenem-resistant NDM variantsAcinetobacter baumanniistrain.
Using PubMed, Web of Science, and Embase databases, we identified all studies addressing the prevalence and variation of NDMAcinetobacter baumanniiisolation. The publications selected for this review were published between 2000 and 2020.
After screening and reviewing the journals, 67 articles finally met the review criteria. The results showed that NDM variants exhibited the greatest diversity in Europe. The prevalence of NDM-1 in Asia, Africa and Europe was 64.02%, 28.05% and 5.56%, respectively. Studies examining genotypes producing NDMAcinetobacter baumanniiMost commonly used strains for PCR.
We've seen an increase in reports of strainsAcinetobacter baumanniiNDMs have been produced on all continents over the past few years, so healthcare providers and physicians as well as microbiologists must pay more attention to preventing the spread of these strains.
SARS-CoV-2 and HIV coinfection; clinical features, diagnosis, and treatment strategies: a systematic review and meta-analysis
2022, Gene Reports
Coronavirus disease 2019 (COVID-19) may be associated with a greater risk of serious complications in immunocompromised human immunodeficiency virus (HIV)-infected individuals. To date, no comprehensive studies have been conducted to assess HIV infection in patients with COVID-19. In this study, we assessed the status of patients coinfected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and HIV through a systematic review and meta-analysis.
A systematic literature search strategy was performed by reviewing original research articles published in Medline, Web of Science and Embase databases in 2019 and 2020. Statistical analyzes reporting HIV prevalence among COVID-19 patients were performed using STATA software version 14.0 (Stata Corporation, College Station, Texas, USA). Case reports/case series were also assessed as systematic reviews.
Our study included 63 studies (53 case reports/case series and 10 prevalence studies). A meta-analysis of prevalence studies showed that six countries (Uganda, China, Iran, USA, Italy, and Spain) reported HIV infection among patients with COVID-19, with an overall frequency of 1.2% [(95% CI) 0.8–1.7] in of 14,424 COVID-19 patients. Based on case reports and case series, among 113 patients with COVID-19 from 19 countries, 111 patients with HIV were reported. Most cases have occurred in the United States, China, Italy and Spain.
The small number of patients with SARS-CoV-2-HIV co-infection reported in the literature makes it difficult to draw precise conclusions. However, because HIV-infected individuals are more likely to develop more severe COVID-19 complications, targeted policies should be considered to address this increased risk in the current pandemic. Our findings highlight the importance of identifying underlying conditions, co-infections, comorbidities, laboratory findings, and beneficial treatment strategies for HIV patients during the COVID-19 pandemic.
Molecular characterization and genetic diversity of clinical isolates of multidrug and extensively drug-resistant Acinetobacter baumannii
2022, Gene Reports
Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen that is a major cause of several infections, including pneumonia, meningitis, blood, wound and urinary tract infections, especially in intensive care units (Zarrilli et al. , 2004; Pogue et al., 2013). The overuse of antibiotics in healthcare settings has led to the expansion of antibiotic resistance and the emergence of multidrug-resistant (MDR), extensively drug-resistant (XDR) and even pan-drug-resistant (PDR) strains (Ghavghani et al., 2019); Rahbarnia et al., 2020; Bahadori et al., 2021). According to a meta-analysis study, the prevalence of MDR in Iran averaged 71%, higher than in other countries, highlighting the urgent need for strict standards to keep the spread of these strains to a minimum (Bialvaei et al., 2017 ).
The increasing prevalence of multidrug-resistant (MDR) strains is now a serious threat to public health. The aim of the study was to investigate the relationship between carbapenemase variants and the spectrum of antibiotic resistanceAcinetobacter baumanniiA strain isolated from Tabriz in northwestern Iran.
127 totalAcinetobacter baumanniiIsolates were obtained from two teaching hospitals in Tabriz, northwest Iran. Multiplex PCR was performed to detect the carbapenemase-encoding gene. In addition, the genetic relationship of the isolates was investigated by Enterobacteriaceae intragenic repeat sequence (ERIC)-PCR technique.
All isolates were resistant to carbapenems based on resistance to meropenem and imipenem. The frequencies of multidrug resistance (MDR) and extensive drug resistance (XDR) were 74.80% and 33.07%, respectively. The most prevalent MDR and XDR were associated with patients younger than 50 years (p<0.05). thisbraOXA-23 sampleGenes were most dominant in 82 (64.5%) of the isolates tested, followed bybraOXA-143 class, 54 (42.51%);braOXA-72, 39 (30.7%). None of the isolates were positivebraOXA-58 classGene. There is a significant relationship between the existence ofbraOXA-23frequency of genes and XDR strains (p<0.01). Based on ERIC-PCR, 88 isolates were clustered into 7 groups, while the remaining 21 were single isolates. Most MDR isolates were associated with clusters B and C (p<0.05). The frequency of OXA-143and OXA-72Significantly higher in A and B clusters (p<0.05). This is the first report of a high incidencebraOXA-143andbraOXA-72positiveAcinetobacter baumanniiIranian pressure. genetic relatednessAcinetobacter baumanniiThe isolates were high, suggesting cross-transmission among hospitalized patients.
High prevalence of MDR and XDRAcinetobacter baumanniiStrains with novel resistance mechanisms have been observed in our region. The incidence of OXA-type carbapenemases varied among clusters classified by ERIC-PCR typing.
Prevalence of biofilm-encoding genes among multidrug-resistant Acinetobacter baumannii isolates
2021, Gene Reports
Furthermore, the isolate was further confirmed by detecting the intrinsic blaOXA-51 gene using specific primers (forward: 5′-ACAAGCGCTATTTTTATTCAG-3′, reverse: 5′-CCCATCCCCAACCACTTTT-3′) (Brossard and Campagnari, 2012). Antibiotic resistance profiles of Acinetobacter baumannii isolates were examined by the Kirby Bauer disk diffusion method according to Clinical Laboratory Standards Institute (CLSI) guidelines (Hudzicki, 2009; Rahbarnia et al., 2020). Antibiotic tablets used were as follows: trimethoprim-sulfamethoxazole (co-trimoxazole) (25 μg), gentamicin (10 μg), ceftazidime (30 μg), amikacin (30 μg) , piperacillin-tazobactam (110 μg), ciprofloxacin (5 μg), lomefloxacin (5 μg), cefotaxime (30 μg), imipenem (10 μg), ceftizole Oxime (30 μg), tigecycline (15 μg) and meropenem (10 μg) antibiotic tablets (MAST, Merseyside, UK).
Increased prevalence of multidrug resistance (MDR)Acinetobacter baumannii isolates coupled with the ability to survive under adverse conditions make this pathogen a serious threat to global health. A cross-sectional study was performed to examine biofilm-forming genes and antibiotic resistance profilesAcinetobacter baumanniiClinical isolates from Tabriz, NW Iran.
Total 100Acinetobacter baumanniiThe strain was isolated from hospitalized patients in the city of Tabriz in northwestern Iran. These isolates were detected by standard microbiological and biochemical testing,braOXA-51detection. After examining the antibiotic resistance profile, the biofilm forming ability was studied by microtiter plate assay. The presencebapandOPGenes related to biofilm formation were detected by PCR reaction.
The highest resistance rates were for meropenem (100%), tigecycline (100%), and ceftizoxime (99%), respectively. Resistance analysis of different antibiotic classes showed that 100% of the isolates were multidrug resistant (MDR) and 79% of them were extensively resistant (XDR). Overall, 84% of the isolates were able to produce biofilms, and 3.57% (3/84) of the isolates had a strong ability to form biofilms. All powerful biofilm formationAcinetobacter baumanniiThe isolate was found to be XDR. Furthermore, 100% of the isolates hadOPOne gene, 89% of which are positivebapGene.
Our findings reveal high prevalence of biofilm formation and biofilm-associated genesbapandOPA multidrug resistanceAcinetobacter baumanniistrains in the study area. According to our results, there is a correlationbapGenes and biofilm formation.
Assessing the Activity of Ultrasound on Acinetobacter baumannii Biofilm Formation in Clinical Specimens
2022, Baghdad Science Journal
Novel strategies for evaluating anticancer molecules of ethno-medicinal plants by in silico and biological approaches--a review
Gene Reports, Volume 18, 2020, Article 100553
Cancer is one of the leading causes of death and is on the rise year by year. Several drugs are available to treat cancer, but none have proven successful. Drug toxicity is one of the major problems in chemotherapy. However, plant and plant-derived drugs have been shown to be effective and safe in the treatment of cancer. Today, most anticancer drug research efforts are derived from plants and plant natural products. Various natural products and their analogs are known to be effective anticancer agents. This review highlights recently known plant species with anticancer properties in vitro or in vivo. The invention of botanical medicines requires a certain amount of time and expense. Currently, several high-throughput techniques have been developed for labor-intensive methods. Bioinformatics and biological sciences play a vital role in the generation of high-throughput data. These are commonly used in drug discovery and design. Currently, bioinformatics and computational methods are rarely used in plant-based research and technologies. In this review, the above research methods of medicinal plants are discussed. Using these methods in medicinal plant-based research may lead to the development of cost-effective and simpler methods for designing plant-based medicines.
Molecular typing of clinical strains of Acinetobacter baumannii by enterobacterial repeat intergenic consensus polymerase chain reaction (ERIC-PCR)
Gene Reports, Volume 18, 2020, Article 100542
Acinetobacter baumanniiIt is one of the most important causes of nosocomial infection. Due to the prevalence and recurrence of infections associated withAcinetobacter baumanniiInfections occurring in different areas of a hospital (eg ICU wards) are important to find the source of infection by different techniques including molecular methods. This study aims to determineAcinetobacter baumanniiIsolation by ERIC-PCR.
In this cross-sectional study, 80Acinetobacter baumanniiIsolates were extracted from patients admitted to the ICU, CCU and post-CCU ward of Ghaem Hospital (Karaj, Iran). After testing the isolates by phenotypic methods, the isolates were first identified using standard biochemical tests and then the API 20NE system kit (Biomerieux, France). Final confirmation was done by PCR tracing the blaOXA-51 gene. Then, the isolates were typed by ERIC-PCR technique. Then the gel electrophoresis images were analyzed by GEL compareII software and the related dendrograms were drawn.
The dendrogram obtained from the study results showed that the isolates were divided into 14 clusters using the ERIC-PCR method.
This study demonstrates that ERIC-PCR technique is a useful tool for studying plant genetic diversity.Acinetobacter baumanniiisolation. return,Acinetobacter baumanniiIsolates were rotated between ICU, CCU and post-CCU at Ghaem Hospital, Karaj, Iran. Also, no specific pattern was observed in the distribution of isolates from one ward to another.
Molecular diversity of hpd genes among clinical isolates of Haemophilus influenzae
Gene Reports, Volume 18, 2020, Article 100556
due to infectionHaemophilus influenzaresulting in enormous global morbidity. Conjugate vaccine againstHaemophilus influenzaeRoutine immunization of infants with type b (Hib) significantly reduces the incidence of invasive Hib disease. Several proteins are used as vaccine candidates for this pathogen, but they do not confer effective immunity against all strains in animal modelsHaemophilus influenzae.This study aims to determine the diversityHDLNucleotide sequences of genes from clinical isolates native to IranHaemophilus influenzaeAs natural vaccine candidates compared to standard strains.
twenty plantsHaemophilus influenzaeRecovered from different clinical specimens from patients admitted to Merad and Imam Khomeini Hospitals, Tehran, Iran. The isolates were then detected and identified asHaemophilus influenzaeusing biochemical tests and further confirmation byomp6Gene polymerase chain reaction. thisHDLThe gene is amplified by PCR using gene-specific primers, and the amplicon isEcoR1. For the four isolates, the ampliconHDLSequence the gene and align the sequence to those in GenBank. Subsequently, the sequences were submitted to the EMBL website (http://www.ebi.ac.uk/embl/).
EcoR1The enzyme digestion zymograms of 19 clinical isolates were the same, only 1 strain was different. Three of the 19 isolates were sequenced. The results showed that the nucleotide sequence and deduced amino acid sequence of protein D among clinical isolates were highly conserved, with >95% similarity.
In conclusion, given the up to 99% similarity among clinical isolates, protein D could be a novel vaccine candidate against all types of viruses.Haemophilus influenzaefrom Iran. This finding should be confirmed with more isolates and the immunological characterization of protein D also evaluated in animal models.
Epidemiology of colistin-resistant, carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in Croatia
Infection, Genetics, and Evolution, Volume 81, 2020, Article 104263
Colistin is an antibiotic of last resort for the treatment of infections caused by multidrug and carbapenem-resistant Gram-negative bacteria. Colistin resistance is emerging, with multiple outbreaks reported in Europe and elsewhere. It is most commonly reported in carbapenem resistanceKlebsiella pneumoniae.In this study, 24 clinical isolates (14Klebsiella pneumoniae, oneEnterobacter aerogenes, oneEnterobacter cloacae,and eightbaumannii)Collected from four hospitals in Croatia from 2013 to 2018 to analyze the molecular epidemiology and mechanisms of antibiotic resistance. β-lactamase and carbapenemase genes were detected by PCR. Genotype selected isolates by repeated PCR. Whole genome sequencing (WGS) was performed to uncover possible molecular mechanisms of the observed colistin resistance. All isolates except twoKlebsiella pneumoniaeisolates with broad drug resistance. 10 out of 16 (63%)Klebsiella pneumoniaeisolated object ownsbraOXA-48,This is the most common carbapenem resistance gene in Croatia and elsewhere in Europe. allAcinetobacter baumanniiIsolates had OXA-23-like carbapenem-hydrolyzing oxacillinases, and five of them were shown to be pan-resistant. Colistin resistance is likely chromosomally mediated. After sequence analysis, no isolates were found to have anycalculusgenetic mutation. Several previously reported mutations associated with colistin resistance were found in PmrB, PhoP, PhoQ, and MgrB. In global phylogenetic analyses, DNA mutations leading to MgrB protein mutations were predominantly found in lineages containing colistin-resistant isolates, and the second most prevalent mutation (K3X) was also encountered in our isolates. Furthermore, based on genotyping by repeated PCR, the spread of colistin resistance was most likely clonal. Most importantly, the presence of colistin resistance and carbapenemase genes in XDR isolates poses a real threat to the use of carbapenems and colistin against infection.
Detection of Carbapenemase, AmpC and ESBL Genes in Acinetobacter ICU by DNA Microarray
Journal of Microbiological Methods, Vol. 155, 2018, Pages 19-23
Accumulation of Multiple Inherent and Acquired Resistance MechanismsAcinetobactergenera leading to the emergence of "pan-resistant" strains, which is one of the major concerns of the global healthcare sector. Monitoring carbapenemase/extended-spectrum beta-lactamases (ESBLs) genesAcinetobacter baumanniiAnalysis by phenotypic methods is challenging, especially in developing countries such as Pakistan. In this case, a novel microarray (CT 103XL Check-MDR) assay was used to simultaneously detect genes encoding clinically important carbapenemases and ESBLs. Results were compared to phenotyping methods including MHT, Rapidec Carba NP, EDTA+DDST, and Rosco (KPC/MBL). Microarray results were also confirmed by PCR. all strainsAcinetobacter baumannii(47) Resistance to imipenem and meropenem. Microarray and PCR results showed OXA-23 was present in all isolatesAcinetobacter baumanniiAnd 36.17% also hold PER. The Rosco kit test showed 100% sensitivity for detecting carbapenemases, but low specificity for classifying them. The Rapidec Carba NP Assay has 100% sensitivity and specificity for the detection of carbapenemases compared to microarrays. 100% sensitivity and specificity for microarray detectionbra-Comparison of genes and PCR. This demonstrates that the Check-MDR CT103 XL assay is an accurate method for identifying ESBLs and carbapenemase genesAcinetobacter baumanniicompared to other methods.
Early detection of metallo-β-lactamase NDM-1 and OXA-23 carbapenemase producing Acinetobacter baumannii in a Libyan hospital
International Journal of Antimicrobial Agents, Vol. 48, No. 1, 2016, Pages 46-50
Acinetobacter baumanniiIt is an opportunistic pathogen that can cause various nosocomial infections. The aim of this study was to characterize the molecular support for carbapenem resistanceAcinetobacter baumanniiClinical isolates recovered from two hospitals in Libya. Bacterial isolates were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing using disk diffusion and Etest methods and investigation of carbapenem resistance determinants by PCR amplification and sequencing. The isolates were subjected to multilocus sequence typing (MLST). All 36 imipenem-resistant strains tested were identified asAcinetobacter baumannii.thisbraOXA-23Genes were detected in 29 strains (80.6%). Metallo-β-lactamasesbraNDM-1Genes were detected in 8 isolates (22.2%), showing the spread of multidrug resistance (MDR)Acinetobacter baumanniiAt the Tripoli Medical Center and the Burn Plastic Surgery Hospital in Libya, including a co-expressionbraOXA-23Gene. MLST reveals several sequence types (ST). Imipenem-resistantAcinetobacter baumanniiST2 was the dominant clone (16/36; 44.4%). This study demonstrates that NDM-1 and OXA-23 contribute to antibiotic resistance in Libyan hospitals and represents the first association of these two carbapenemases in native MDRAcinetobacter baumanniiIsolation from a patient in Libya suggests chronic infection control problems in these hospitals.
© 2019 Elsevier plc. all rights reserved.
Acinetobacter baumannii is a frequent cause of the nosocomial infections. Herein, a novel isothermal amplification technique, multiple cross displacement amplification (MCDA) is employed for detecting all A. baumannii strains and identifying the strains harboring blaOXA-23-like gene.What are the symptoms of carbapenem-resistant Acinetobacter? ›
Carbapenem-resistant Acinetobacter cause pneumonia and wound, bloodstream, and urinary tract infections. These infections tend to occur in patients in intensive care units. Carbapenem-resistant Acinetobacter can carry mobile genetic elements that are easily shared between bacteria.What infections are caused by carbapenem-resistant Acinetobacter baumannii? ›
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a type of bacteria commonly found in the environment, especially in soil and water. CRAB can cause human infections of the blood, urinary tract, lungs, wounds, and other body sites. The bacteria are multidrug-resistant, making infections very difficult to treat.Is Acinetobacter baumannii in urinary infection? ›
Acinetobacter baumannii can cause infections in the blood, urinary tract, and lungs (pneumonia), or in wounds in other parts of the body. It can also “colonize” or live in a patient without causing infections or symptoms, especially in respiratory secretions (sputum) or open wounds.How to test for carbapenem resistant Acinetobacter baumannii? ›
Screening by rectal swab or stool specimen has 61%–99% sensitivity to detect VRE,8 and 76%–100% sensitivity to detect CRE. An important issue to consider before implementing a screening policy is the pretest probability of screening positive.Where is Acinetobacter baumannii most commonly found? ›
Acinetobacter baumannii is a nosocomial pathogen, with cases of infection with the organism being found almost exclusively in hospital patients. Unlike the ubiquitous Acinetobacter spp., A. baumannii rarely has been found on human skin.How serious is Acinetobacter? ›
Acinetobacter can live on or in a patient without causing infection or symptoms, but can also potentially cause a variety of diseases, including lung infections (“pneumonia”) and blood, wound, or urinary tract infections. Typical symptoms of pneumonia could include fever, chills, or cough.Can Acinetobacter be cured? ›
The clinical cure rates with imipenem for ventilator-associated pneumonia due to Acinetobacter range from 57 to 83 percent in small series [46-48,60]. For bacteremia, one small case series reported successful clinical outcome in 56 percent of individuals treated with imipenem .How do you treat carbapenem-resistant Acinetobacter? ›
Ampicillin-sulbactam has been used successfully to treat invasive infections caused by A. baumannii strains , with sulbactam being the active component of this combination against some carbapenem-resistant strains .What are the signs and symptoms of Acinetobacter baumannii? ›
- Red, swollen, warm, or painful skin areas or wounds.
- An area of orange, bumpy skin with blisters.
- Cough, chest pain, or trouble breathing.
- Burning feeling while you urinate.
- Sleepiness, headaches, or a stiff neck.
Acinetobacter baumannii, primarily a nosocomial pathogen, is one of the most antibiotic resistant pathogens in clinical medicine (1). Estimates of mortality rates among patients with A. baumannii infections have ranged from 26.0% to 55.7%, with estimated attributable mortality rates between 8.4% and 36.5% (2).Is carbapenem resistant Acinetobacter contagious? ›
CRAB is most often spread person-to-person in health care through direct contact with infected or colonized residents, via health care workers' hands following care of those residents, or from the bacteria's persistence in the resident's environment.How do you treat Acinetobacter baumannii infection? ›
Owing to the propensity of Acinetobacter to develop resistance to antibiotics, current treatment strategies remain limited. Beta-lactam antibiotics are the preferred antibacterial choices for susceptible A baumannii infections.How long is treatment for Acinetobacter UTI? ›
But, there have been more recent outbreaks with extensively drug-resistant Acinetobacter which makes the management of these infections much more complicated.  The duration of therapy is from 7 to 10 days, depending on the patient illness.Can Acinetobacter baumannii cause sepsis? ›
baumannii isolates (4,–7) whose resistance allows them to disseminate, giving rise to septic shock and disseminated intravascular coagulation (DIC). Consequently, mortality rates range from 30 to 75% depending on the route of infection (8).What is the best antibiotic for Acinetobacter baumannii? ›
In 2019, Assimakopoulos et al. reported positive results in treating 10 ICU patients with VAP from Pan Drug-Resistant Acinetobacter baumannii with a combination of antibiotics, which consisted of a high dose of tigecycline and ampicillin/sulbactam, and colistin, given both by inhalation and intravenously (22).What does Acinetobacter baumannii UTI cause? ›
baumannii causes infections of the urinary tract, usually associated with the presence of percutaneous nephrostomy tubes or urinary catheters. These pathogenic strains are also responsible for meningitis, osteomyelitis, endocarditis and wound infections.Which is the most recommended test for detection of carbapenemase production? ›
Modified Hodge test
Carbapenemase producing isolate is detected by the MHT when the test isolate produces the enzyme and allows the growth of the carbapenem susceptible E. coli ATCC 25922 strain towards the disk.
Strictly aerobic, Acinetobacter spp. grow on most routinely used media at temperatures of 20–44 °C (68–111 °F). Acinetobacter spp. isolated from human specimens grow readily at 37 °C (99 °F).What food sources does Acinetobacter come from? ›
Acinetobacter baumannii has been isolated from soil, water and foods including meat, fish, fruits and vegetables, raising concerns food could be a potential source of infection to humans, particularly in healthcare settings. Acinetobacter includes a group of bacteria commonly found in soil and water.
The superbug in question is Acinetobacter baumannii, which the World Health Organization has classified as a “critical” threat among its “priority pathogens” – a group of bacteria families that pose the “greatest threat” to human health.How long does Acinetobacter live? ›
baumannii can survive for 6 days on dry filter paper (1), 13 days on formica (12, 19), more than 7 days on glass (16), and more than 25 days on cotton (16).What is the survival time on surfaces of Acinetobacter? ›
The mean survival times of the various A. baumannii isolates tested are shown in Tables 1 and 2. The mean survival time for sporadic strains was 27.29 days (range, 21 to 32 days), while the mean survival time for outbreak strains was 26.55 days (range, 21 to 33 days).Is Acinetobacter normal? ›
Acinetobacter baumannii is not part of the normal human skin or gut flora and is usually not carried by a previously healthy individual.Is Acinetobacter airborne? ›
Infected patients spreads Acinetobacter to the air of intensive care unit (ICU). These strains can remain in ICU air about four weeks.Why is Acinetobacter important? ›
The ability of Acinetobacter strains to adhere to surfaces is an important mechanism in the pathogenicity. It frequently causes infections associated with medical devices, e.g., vascular catheters, cerebrospinal fluid shunts or Foley catheters. Biofilm formation is a well-known pathogenic mechanism in such infections.What is the isolation for Acinetobacter? ›
Isolation of Acinetobacter from colonized patients requires no specific therapy. Acinetobacter isolates demonstrate increasing resistance to commonly prescribed antimicrobials.What is the mortality rate of carbapenem resistant Acinetobacter? ›
Among patients with CR A. baumannii infections, the highest mortality rate was observed for bloodstream infections (40.9%; Table 3) followed by respiratory tract infections (21.9%), while mortality was 9.3% for patients with urinary tract infections and 6.6% for those with wound infections (Table 3).Why is A. baumannii difficult to treat? ›
Mechanisms of Resistance. The management of AB infections is particularly complicated due to this pathogen's multiple intrinsic and acquired mechanisms of resistance including β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, permeability defects, and target site modifications.How do you get carbapenem resistant organism? ›
Typically patients with CRE infections have a history of long term exposure to health care facilities usually due to unrelated comorbidity. This exposure usually coincides with long term and varied antibiotic use leading to the gradual development of more and more resistant bacterial infections.
Carbapenem has a five-membered ring, as does penicillin, but it has a carbon at C-1 instead of sulfur.What kills Acinetobacter? ›
Highly antibiotic-resistant Acinetobacter baumannii clinical isolates are killed by the green tea polyphenol (–)-epigallocatechin-3-gallate (EGCG)Does Acinetobacter cause endocarditis? ›
Infective endocarditis caused by Acinetobacter (A.) baumannii is a rare but severe complication that affects seriously ill, hospitalized patients undergoing invasive procedures.Which media is used for Acinetobacter baumannii? ›
MacConkey agar was 89% sensitive for Acinetobacter species and 91% sensitive for MDR-A. baumannii when compared to sheep blood agar.What are the identifying characteristics of Acinetobacter species? ›
Characteristics. Acinetobacters are short, plump rods, typically measuring 1.0–1.5 × 1.5–2.5 μm when in the logarithmic phase of growth, but they often become more coccoid in the stationary phase. They are Gram-negative but may appear Gram variable, as is typical of members of the Moraxellaceae generally.How do you determine antibacterial susceptibility test? ›
The Kirby-Bauer agar diffusion method is well documented and is the standardized method for determining antimicrobial susceptibility. White filter paper disks (6 mm in diameter) are impregnated with known amounts of antimicrobial agents. Each disk is coded with the name and concentration of the agent.What are the distinguishing characteristics of Acinetobacter baumannii? ›
baumannii is one of the most challenging bacterial pathogens because of its unique antibiotic resistance characteristics. The genus Acinetobacter includes non-lactose-fermenting, catalase-positive, non-motile, non-fastidious, oxidase-negative, and aerobic Gram-negative coccobacilli.How do you treat Acinetobacter baumannii skin infection? ›
First-line antibiotics — Infections caused by antibiotic-susceptible Acinetobacter isolates may have several first-line therapeutic options, including broad-spectrum cephalosporins (ceftazidime or cefepime), piperacillin-tazobactam, ampicillin-sulbactam, carbapenems (eg, meropenem or imipenem-cilastatin), and ...Is Acinetobacter baumannii positive or negative? ›
Acinetobacter baumannii is a Gram-negative bacillus that is aerobic, pleomorphic and non-motile. An opportunistic pathogen, A. baumannii has a high incidence among immunocompromised individuals, particularly those who have experienced a prolonged (> 90 d) hospital stay.What are the signs and symptoms of Acinetobacter infection? ›
Symptoms of a bloodstream infection might include fever, chills, vomiting, and confusion. A wound infection might cause fever and redness, increasing pain, and pus around the wound.
Isolation of Acinetobacter from colonized patients requires no specific therapy. Acinetobacter isolates demonstrate increasing resistance to commonly prescribed antimicrobials. Resistance has been tracked to plasmids, transposons and chromosomes.What is the confirmation test for Acinetobacter? ›
The negative oxidase test is important for rapid presumptive identification to differentiate the genus Acinetobacter from other similar non-fermentative organisms (Joshi and Litake, 2013). ...What is the strongest antibiotic for bacterial infection? ›
Vancomycin 3.0 is one of the most potent antibiotics ever created.What do susceptibility test results mean? ›
A “susceptible” result indicates that the patient's organism should respond to therapy with that antibiotic using the dosage recommended normally for that type of infection and species [13, 20].How do you treat antibiotic resistant bacteria? ›
Sometimes doctors prescribe a combination of medications. Treatment of a MRSA infection at home often involves a 7- to 10-day course of an antibiotic such as clindamycin, doxycycline, or a combination of sulfamethoxazole–trimethoprim and linezolid.What are two Acinetobacter species of clinical importance? ›
Clinically relevant species are mostly confined to the Acinetobacter calcoaceticus/Acinetobacter baumannii (ACB) complex, namely, A. baumannii, A. calcoaceticus, Acinetobacter pittii, Acinetobacter nosocomialis, and the recently added numerous other species [3,4,5,6]. However, among the ACB complex, A.What is the difference between Acinetobacter baumannii colonization and infection? ›
baumannii colonization means sputum culture from lower respiratory tract is positive without infection signs. And A. baumannii infection means sputum culture from lower respiratory tract is positive with infection signs.